Diffraction phase microscopy with white light.

نویسندگان

  • Basanta Bhaduri
  • Hoa Pham
  • Mustafa Mir
  • Gabriel Popescu
چکیده

We present white light diffraction phase microscopy (wDPM) as a quantitative phase imaging method that combines the single shot measurement benefit associated with off-axis methods, high temporal phase stability associated with common path geometries, and high spatial phase sensitivity due to the white light illumination. We propose a spatiotemporal filtering method that pushes the limit of the pathlength sensitivity to the subangstrom level at practical spatial and temporal bandwidths. We illustrate the utility of wDPM with measurements on red blood cell morphology and HeLa cell growth over 18 hours.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

White-light diffraction phase microscopy at doubled space-bandwidth product.

White light diffraction microscopy (wDPM) is a quantitative phase imaging method that benefits from both temporal and spatial phase sensitivity, granted, respectively, by the common-path geometry and white light illumination. However, like all off-axis quantitative phase imaging methods, wDPM is characterized by a reduced space-bandwidth product compared to phase shifting approaches. This happe...

متن کامل

Translucent tile construction and its properties

Translucent tiles are among the tiles that have recently been featured. Passing part of the light is an important feature of these tiles. The passage of light from within, create a beautiful effect. In particular, it can be combined with LED, in which its beauty will be multiplied. In this research, the effect of using transparent frit and feldspar on porcelain tile composition was studied to o...

متن کامل

White-light interference microscopy: minimization of spurious diffraction effects by geometric phase-shifting.

A common problem when profiling surfaces with steps or discontinuities using white-light (coherence-probe) interferometry is localized spikes (batwings) or spurious peaks due to diffraction effects. We show that errors due to these effects can be minimized by processing the irradiance data obtained with an achromatic phase-shifter operating on the geometric (Pancharatnam) phase to yield the val...

متن کامل

Instantaneous Spatial Light Interference Microscopy.

We present Instantaneous Spatial Light Interference Microscopy (iSLIM) as a new quantitative phase method that combines the benefits of white light illumination in Zernike's phase contrast microscopy and phase stability associated diffraction phase microscopy. iSLIM is implemented as an add-on module to a commercial phase contrast microscope, and enables new features to quantitative phase imagi...

متن کامل

White-light diffraction tomography of unlabelled live cells

We present a technique called white-light diffraction tomography (WDT) for imaging microscopic transparent objects such as live unlabelled cells. The approach extends diffraction tomography to white-light illumination and imaging rather than scattering plane measurements. Our experiments were performed using a conventional phase contrast microscope upgraded with a module to measure quantitative...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Optics letters

دوره 37 6  شماره 

صفحات  -

تاریخ انتشار 2012